Hi,

--- UPDATE ---
I tricked unicycler to work with version 3.14.1 editing the misc.py file and it outputs an assemblage. Can I trust it ?

Is there a way to use unicycler with Spades version 3.14 ? It is the only version that do not crash on my data for an obscure reason. I tried to start at read error correction or at assembly without correction and I always get this error.

Thank you very much in advance,

One spades log file for example :
`Command line: /home/oar-jobs/tools/src/SPAdes-versions/SPAdes-3.12.0-Linux/bin/spades.py -1 /tshared/data/Genomics_rawdata/2020-12-08_DY0_DY1_M4_MegaPleistocene_MimiRiviere_illumina/CedratDY1/DY1_FDSW202615984-1r_HJVTGDSXY_L2_1.fq.gz -2 /tshared/data/Genomics_rawdata/2020-12-08_DY0_DY1_M4_MegaPleistocene_MimiRiviere_illumina/CedratDY1/DY1_FDSW202615984-1r_HJVTGDSXY_L2_2.fq.gz -o /home/rigou/Cedrats_Siberi2020/CedratDY12020_unicycler/spades_assembly/read_correction --threads 32 --only-error-correction --phred-offset 33

System information:
SPAdes version: 3.12.0
Python version: 2.7.16
OS: Linux-4.19.0-10-amd64-x86_64-with-debian-10.7

Output dir: /home/rigou/Cedrats_Siberi2020/CedratDY12020_unicycler/spades_assembly/read_correction
Mode: ONLY read error correction (without assembling)
Debug mode is turned OFF

Dataset parameters:
Multi-cell mode (you should set '--sc' flag if input data was obtained with MDA (single-cell) technology or --meta flag if processing metagenomic dataset)
Reads:
Library number: 1, library type: paired-end
orientation: fr
left reads: ['/tshared/data/Genomics_rawdata/2020-12-08_DY0_DY1_M4_MegaPleistocene_MimiRiviere_illumina/CedratDY1/DY1_FDSW202615984-1r_HJVTGDSXY_L2_1.fq.gz']
right reads: ['/tshared/data/Genomics_rawdata/2020-12-08_DY0_DY1_M4_MegaPleistocene_MimiRiviere_illumina/CedratDY1/DY1_FDSW202615984-1r_HJVTGDSXY_L2_2.fq.gz']
interlaced reads: not specified
single reads: not specified
merged reads: not specified
Read error correction parameters:
Iterations: 1
PHRED offset: 33
Corrected reads will be compressed
Other parameters:
Dir for temp files: /home/rigou/Cedrats_Siberi2020/CedratDY12020_unicycler/spades_assembly/read_correction/tmp
Threads: 32
Memory limit (in Gb): 250

======= SPAdes pipeline started. Log can be found here: /home/rigou/Cedrats_Siberi2020/CedratDY12020_unicycler/spades_assembly/read_correction/spades.log

===== Read error correction started.

== Running read error correction tool: /home/oar-jobs/tools/src/SPAdes-versions/SPAdes-3.12.0-Linux/bin/spades-hammer /home/rigou/Cedrats_Siberi2020/CedratDY12020_unicycler/spades_assembly/read_correction/corrected/configs/config.info

The program was terminated by segmentation fault
=== Stack Trace ===

== Error == system call for: "['/home/oar-jobs/tools/src/SPAdes-versions/SPAdes-3.12.0-Linux/bin/spades-hammer', '/home/rigou/Cedrats_Siberi2020/CedratDY12020_unicycler/spades_assembly/read_correction/corrected/configs/config.info']" finished abnormally, err code: -11

In case you have troubles running SPAdes, you can write to spades.support@cab.spbu.ru
`