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Hi Felipe,
Could you help with this issue I've got when running bacannot everything went just fine except the pipeline arrives that step. I tried twice with differents genomes and it fails at the same point, SUMMARY.
Command line
$ nextflow run fmalmeida/bacannot --input bacannot_samplesheet.yaml --output Resultadosilvestres --bacannot_db ./bacannot_db_2023 --max_memory 100.GB -profile docker
Launching
$ nextflow run fmalmeida/bacannot --input bacannot_samplesheet.yaml --output Resultadosilvestres --bacannot_db ./bacannot_db_2023 --max_memory 100.GB -profile docker
N E X T F L O W ~ version 23.04.1
Launching `https://github.com/fmalmeida/bacannot` [big_lamport] DSL2 - revision: 0798f3ce1e [master]
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fmalmeida/bacannot v3.2
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Core Nextflow options
revision : master
runName : big_lamport
containerEngine: docker
launchDir : /home/jfrancisco
workDir : /home/jfrancisco/work
projectDir : /home/jfrancisco/.nextflow/assets/fmalmeida/bacannot
userName : jfrancisco
profile : docker
configFiles : /home/jfrancisco/.nextflow/assets/fmalmeida/bacannot/nextflow.config
Input/output options
input : bacannot_samplesheet.yaml
output : Resultadosilvestres
bacannot_db : ./bacannot_db_2023
Max job request options
max_memory : 100.GB
!! Only displaying parameters that differ from the pipeline defaults !!
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If you use fmalmeida/bacannot for your analysis please cite:
* The pipeline
https://doi.org/10.5281/zenodo.3627669
* The nf-core framework
https://doi.org/10.1038/s41587-020-0439-x
* Software dependencies
https://github.com/fmalmeida/bacannot/blob/master/CITATIONS.md
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executor > local (1687)
[- ] process > BACANNOT:UNICYCLER -
[- ] process > BACANNOT:FLYE -
[a1/448c55] process > BACANNOT:PROKKA (sample_2262) [100%] 73 of 73 ✔
[2a/b5ccb5] process > BACANNOT:MLST (sample_2262) [100%] 73 of 73 ✔
[27/4a09df] process > BACANNOT:BARRNAP (sample_2262) [100%] 73 of 73 ✔
[6e/2d8836] process > BACANNOT:COMPUTE_GC (sample_2262) [100%] 73 of 73 ✔
[70/1d73a2] process > BACANNOT:KOFAMSCAN (sample_2262) [100%] 73 of 73 ✔
[e6/b6657d] process > BACANNOT:KEGG_DECODER (sample_2262) [100%] 73 of 73 ✔
[08/6b5271] process > BACANNOT:PLASMIDFINDER (sample_2262) [100%] 73 of 73 ✔
[07/496d95] process > BACANNOT:PLATON (sample_2262) [100%] 73 of 73 ✔
[da/a90301] process > BACANNOT:ISLANDPATH (sample_2262) [100%] 73 of 73 ✔
[f2/071924] process > BACANNOT:VFDB (sample_2262) [100%] 73 of 73 ✔
[0e/8bc7f5] process > BACANNOT:VICTORS (sample_2262) [100%] 73 of 73 ✔
[7e/b3f901] process > BACANNOT:PHAST (sample_2262) [100%] 73 of 73 ✔
[85/27ae8e] process > BACANNOT:PHIGARO (sample_2262) [100%] 73 of 73 ✔
[d7/916c42] process > BACANNOT:PHISPY (sample_2262) [100%] 73 of 73 ✔
[41/79fa26] process > BACANNOT:ICEBERG (sample_2262) [100%] 73 of 73 ✔
[63/004f99] process > BACANNOT:AMRFINDER (sample_2262) [100%] 73 of 73 ✔
[02/91eff0] process > BACANNOT:CARD_RGI (sample_2262) [100%] 73 of 73 ✔
[61/ec5110] process > BACANNOT:ARGMINER (sample_2262) [100%] 73 of 73 ✔
[- ] process > BACANNOT:RESFINDER -
executor > local (1687)
[- ] process > BACANNOT:UNICYCLER -
[- ] process > BACANNOT:FLYE -
[a1/448c55] process > BACANNOT:PROKKA (sample_2262) [100%] 73 of 73 ✔
[2a/b5ccb5] process > BACANNOT:MLST (sample_2262) [100%] 73 of 73 ✔
[27/4a09df] process > BACANNOT:BARRNAP (sample_2262) [100%] 73 of 73 ✔
[6e/2d8836] process > BACANNOT:COMPUTE_GC (sample_2262) [100%] 73 of 73 ✔
[70/1d73a2] process > BACANNOT:KOFAMSCAN (sample_2262) [100%] 73 of 73 ✔
[e6/b6657d] process > BACANNOT:KEGG_DECODER (sample_2262) [100%] 73 of 73 ✔
[08/6b5271] process > BACANNOT:PLASMIDFINDER (sample_2262) [100%] 73 of 73 ✔
[07/496d95] process > BACANNOT:PLATON (sample_2262) [100%] 73 of 73 ✔
[da/a90301] process > BACANNOT:ISLANDPATH (sample_2262) [100%] 73 of 73 ✔
[f2/071924] process > BACANNOT:VFDB (sample_2262) [100%] 73 of 73 ✔
[0e/8bc7f5] process > BACANNOT:VICTORS (sample_2262) [100%] 73 of 73 ✔
[7e/b3f901] process > BACANNOT:PHAST (sample_2262) [100%] 73 of 73 ✔
[85/27ae8e] process > BACANNOT:PHIGARO (sample_2262) [100%] 73 of 73 ✔
[d7/916c42] process > BACANNOT:PHISPY (sample_2262) [100%] 73 of 73 ✔
[41/79fa26] process > BACANNOT:ICEBERG (sample_2262) [100%] 73 of 73 ✔
[63/004f99] process > BACANNOT:AMRFINDER (sample_2262) [100%] 73 of 73 ✔
[02/91eff0] process > BACANNOT:CARD_RGI (sample_2262) [100%] 73 of 73 ✔
[61/ec5110] process > BACANNOT:ARGMINER (sample_2262) [100%] 73 of 73 ✔
[- ] process > BACANNOT:RESFINDER -
[- ] process > BACANNOT:CALL_METHYLATION -
[d5/04ae86] process > BACANNOT:REFSEQ_MASHER (sample_2262) [100%] 73 of 73 ✔
[0c/ed95c8] process > BACANNOT:DIGIS (sample_2340) [100%] 73 of 73 ✔
[42/e619d9] process > BACANNOT:ANTISMASH (sample_2262) [100%] 73 of 73 ✔
[d5/578449] process > BACANNOT:SEQUENCESERVER (sample_2262) [100%] 73 of 73 ✔
[89/c0fe76] process > BACANNOT:MERGE_ANNOTATIONS (sample_2262) [100%] 73 of 73 ✔
[54/ee598b] process > BACANNOT:DRAW_GIS (sample_139) [ 2%] 2 of 68
[94/c0c5ca] process > BACANNOT:GFF2GBK (sample_172) [ 4%] 3 of 68
[- ] process > BACANNOT:CREATE_SQL [ 0%] 0 of 68
[- ] process > BACANNOT:JBROWSE -
[- ] process > BACANNOT:REPORT -
[e1/96ee86] process > BACANNOT:SUMMARY (sample_160) [ 1%] 1 of 68, failed: 1
[- ] process > BACANNOT:MERGE_SUMMARIES -
[4b/115153] process > BACANNOT:CIRCOS (sample_139) [ 2%] 2 of 68
Execution cancelled -- Finishing pending tasks before exit
ERROR ~ Error executing process > 'BACANNOT:SUMMARY (sample_160)'
Caused by:
Process `BACANNOT:SUMMARY (sample_160)` terminated with an error exit status (1)
Command executed:
mkdir -p results/sample_160/annotation
ln -rs annotation/* results/sample_160/annotation
source activate falmeida-py
falmeida-py bacannot2json -i results -o sample_160_summary.json
Command exit status:
1
Command output:
(empty)
Command error:
Traceback (most recent call last):
File "/opt/conda/envs/falmeida-py/bin/falmeida-py", line 33, in <module>
sys.exit(load_entry_point('falmeida-py==1.0.0', 'console_scripts', 'falmeida-py')())
File "/opt/conda/envs/falmeida-py/lib/python3.7/site-packages/falmeida_py/__main__.py", line 212, in main
bacannot2json(args['--input'], args['--output'], args['--print'])
File "/opt/conda/envs/falmeida-py/lib/python3.7/site-packages/falmeida_py/bacannot2json.py", line 107, in bacannot2json
plasmids_stats( bacannot_summary )
File "/opt/conda/envs/falmeida-py/lib/python3.7/site-packages/falmeida_py/plasmid_function.py", line 73, in plasmids_stats
bacannot_summary[sample]['plasmid']['plasmidfinder']['meta']['database'] = results['Database'].unique().item()
ValueError: can only convert an array of size 1 to a Python scalar
Work dir:
/home/jfrancisco/work/e1/96ee86a42df4c84ef36c2bc0c22b82
Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
-- Check '.nextflow.log' file for details
Thanks for all.
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