Hi Alex,

I'm getting Segmentation fault: 11 immediatly after mapping started. Below is the code I run and the error I got:

STAR --runThreadN 8 --genomeDir /Volumes/som-ts/GynOb/SmithLab/NewData/People/Seyma/RNAseqRefGenomeGRCh38_100Reads --sjdbGTFfile ~/Downloads/gencode.v28.primary_assembly.annotation.gtf --readFilesIn /Volumes/som-ts/GynOb/SmithLab/NewData/Projects/PTSD/RawFiles/BEAR/RNASeq/ASM12669-76420/HNHVVDSX2_s1_1_IDT8_UDI_028_i7-IDT8_UDI_028_i5_6771-EE-0095.fastq.gz /Volumes/som-ts/GynOb/SmithLab/NewData/Projects/PTSD/RawFiles/BEAR/RNASeq/ASM12669-76420/HNHVVDSX2_s1_2_IDT8_UDI_028_i7-IDT8_UDI_028_i5_6771-EE-0095.fastq.gz --readFilesCommand gunzip -c --quantMode GeneCounts --outSAMtype BAM SortedByCoordinate --outBAMsortingThreadN 1

STAR version: 2.7.10a compiled: :/Users/skatrin/Downloads/STAR-2.7.10a/source
Jan 25 16:34:34 ..... started STAR run
Jan 25 16:34:34 ..... loading genome
Jan 25 16:39:56 ..... processing annotations GTF
Jan 25 16:40:27 ..... inserting junctions into the genome indices
Jan 25 16:43:43 ..... started mapping
Segmentation fault: 11

I'm running it on a Mac with 32 GB ram. The reads are 100 bp Illumina Pair End reads. The ref genome is human GRCh38.p12. Here is the code that I used to create the genome index (maybe that's the problem?).

STAR --runThreadN 8
--runMode genomeGenerate
--genomeDir /Volumes/som-ts/GynOb/SmithLab/NewData/People/Seyma/RNAseqRefGenomeGRCh38_100Reads
--genomeFastaFiles ~/Downloads/GRCh38.primary_assembly.genome.fa
--sjdbGTFfile ~/Downloads/gencode.v28.primary_assembly.annotation.gtf
--sjdbOverhang 100
--genomeChrBinNbits 15
--limitGenomeGenerateRAM 24000000000

Log file is attached. Any help would be much appreciated!
Log.out.txt