Hi there,

While doing an all-to-all ANI comparison on a set of genomes, I noticed that the regular output displays different values when the genomes are switched:

1509405_PRJNA252589.fasta.gz  246200_PRJNA281.fasta.gz      76.3461  98    1679
246200_PRJNA281.fasta.gz      1509405_PRJNA252589.fasta.gz  76.9103  84    1369

When using the --matrix option there is only a single value for this pair, which is 76.628181 (looks like the mean).

I thus have two questions:

• Why do the ANI values change when the genome are switched?
• Is there a particular reason to use the mean of the two values in the --matrix output?

Cheers,
Nils